Coding

Part:BBa_K1367002:Design

Designed by: Agatha Mia   Group: iGEM14_UB_Indonesia   (2014-10-08)


TALE-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 478
    Illegal NheI site found at 700
    Illegal NheI site found at 1144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
    Illegal XhoI site found at 829
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1216
    Illegal BsaI.rc site found at 286
    Illegal BsaI.rc site found at 610
    Illegal BsaI.rc site found at 832


Design Notes

One of the difficulties of this design is to match the sequence in the plasmid pSB1C3


Source

The source of this part came from GATE Assembly Kit from BIOSS in University of Freiburg in Germany

References